Silk fm dating
Cell based restoration of RPE cells has been explored in several studies.
Implantation of RPE as an intact cell sheet should in theory prevent the mentioned disadvantages of injecting RPE.
Cutting down the time necessary for producing differentiated RPE reduces costs of production and lessens the risk of infection during prolonged cultures.
The silk protein sericin mainly consist of three polypeptides having molecular masses of 400, 250, and 150 k Da.
Increased levels of RPE-associated proteins (RPE65 and the pigment melanin) in the sericin-supplemented cultures were confirmed by western blot, spectrophotometry and transmission electron microscopy.
Sericin also increased cell density and reduced cell death following serum starvation in culture.
Inclusion of NF-κB agonists and antagonists in the culture medium showed that activation of the NF-κB pathway appears to be necessary, but not sufficient, for sericin-induced RPE pigmentation.
The disorder is subdivided into a dry and a wet type.
There are no established curative treatment options for the dry type of AMD, which constitutes more than 85% of the cases.
Complement component 3 (C3) was the second most up-regulated gene in the sericin group, with a 34.7-fold up-regulation.